Separase Polyclonal Antibody

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Home > Product > Antibody > Separase Polyclonal Antibody

Separase Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Separase Antibody

Code

BT-AP08213

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human SEPARASE. AA range:767-816

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids.

Uniprot accession

Human:;Mouse:;

Molecular weight

233113

Gene ID

Human: 9700; Mouse: 105988

Synonyms

ESPL1; ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase

Research area

Cell Biology

Target protein

ESPL1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 40000

Images

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SEPARASE Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HUVEC cells, using SEPARASE Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Separase Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from 293 cells, treated with EGF 200ng/ml 30', using SEPARASE Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of A431 cells using Separase Polyclonal Antibody diluted at 1:1000

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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