SPARC Polyclonal Antibody

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Home > Product > Antibody > SPARC Polyclonal Antibody

SPARC Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

SPARC Antibody

Code

BT-AP08493

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human SPARC. AA range:141-190

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

SPARC encodes a cysteine-rich acidic matrix-associated protein. The secreted protein acidic and cysteine rich is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

33330

Gene ID

Human: 6678; Mouse: 20692; Rat: 24791

Synonyms

SPARC; ON; SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine

Research area

Cell Biology

Target protein

SPARC

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 20000

Images

Immunohistochemical analysis of paraffin-embedded mouse-muscle, antibody was diluted at 1:100

Western Blot analysis of HeLa, HepG2 cells using SPARC Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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