TGase2 Polyclonal Antibody

Product overview

Full product name

TGase2 Antibody

Code

BT-AP08965

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Transglutaminase 2. AA range:1-50

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. Protein-glutamine gamma-glutamyltransferase 2 encoded by TGM2 acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the protein-glutamine gamma-glutamyltransferase 2 is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

Human:;Mouse:;

Molecular weight

77329

Gene ID

Human: 7052; Mouse: 21817

Synonyms

TGM2; Protein-glutamine gamma-glutamyltransferase 2; Tissue transglutaminase; Transglutaminase C; TG(C); TGC; TGase C; Transglutaminase H; TGase H; Transglutaminase-2; TGase-2

Research area

Cell Biology

Target protein

TGM2

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 20000

Images

Immunohistochemical analysis of paraffin-embedded Human placenta. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Endometrium. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Transglutaminase 2 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human placenta. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Western Blot analysis of various cells using TGase2 Polyclonal Antibody

Western blot analysis of lysates from HUVEC cells, using Transglutaminase 2 Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

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