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Home > Product > Antibody > TIF1 Alpha Polyclonal Antibody

TIF1 Alpha Polyclonal Antibody

TIF1 Alpha Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-p, ELISA

Product overview

Full product name

TIF1alpha Antibody

Code

BT-AP09021

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human TRIM24. AA range:1001-1050

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Transcription intermediary factor 1-alpha encoded by TRIM24 mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Uniprot accession

Molecular weight

116831

Gene ID

Human: 8805; Mouse: 21848

Synonyms

TRIM24; RNF82; TIF1; TIF1A; Transcription intermediary factor 1-alpha; TIF1-alpha; E3 ubiquitin-protein ligase TRIM24; RING finger protein 82; Tripartite motif-containing protein 24

Research area

Signal Transduction

Target protein

TRIM24

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
ELISA 1: 20000

Images

Western Blot analysis of COLO205 cells using TIF1α Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
Western blot analysis of the lysates from HepG2 cells using TRIM24 antibody.
Western blot analysis of lysates from COLO cells, using TRIM24 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TRIM24 Antibody. The picture on the right is blocked with the synthesized peptide.
Western Blot analysis of various cells using TIF1α Polyclonal Antibody diluted at 1:1000

Protocols

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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