Troponin I-C Polyclonal Antibody

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Home > Product > Antibody > Troponin I-C Polyclonal Antibody

Troponin I-C Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Troponin I-C Antibody

Code

BT-AP09225

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from mouse TNNI3. AA range:5-54

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

Uniprot accession

Human:;Rat:;

Molecular weight

24008

Gene ID

Mouse: 21954; Rat: 29248

Synonyms

TNNI3; TNNC1; Troponin I; cardiac muscle; Cardiac troponin I

Research area

Others

Target protein

TNNI3

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 5000

Images

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using TNNI3 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of HepG2 cells, using TNNI3 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from mouse heart cells, using TNNI3 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of mouse cells using Troponin I-C Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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