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Home > Product > Antibody > UBF-1 Polyclonal Antibody

UBF-1 Polyclonal Antibody

UBF-1 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

UBF-1 Antibody

Code

BT-AP09369

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human UBF1. AA range:501-550

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

UBTF encodes a member of the HMG-box DNA-binding protein family. The nucleolar transcription factor 1 plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11.

Uniprot accession

Molecular weight

89406

Gene ID

Human: 7343; Rat: 25574

Synonyms

UBTF; UBF; UBF1; Nucleolar transcription factor 1; Autoantigen NOR-90; Upstream-binding factor 1; UBF-1

Research area

Epigenetics and Nuclear Signaling

Target protein

UBTF

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
ELISA 1: 20000

Images

Western Blot analysis of Jurkat cells using UBF-1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus.
Western Blot analysis of various cells using UBF-1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using UBF1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa and Jurkat cells, using UBF1 Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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