Vinculin Polyclonal Antibody

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Home > Product > Antibody > Vinculin Polyclonal Antibody

Vinculin Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Vinculin Antibody

Code

BT-AP09531

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Vinculin. AA range:786-835

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for VCL, but the biological validity of some variants has not been determined.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

123799

Gene ID

Human: 7414; Mouse: 22330; Rat: 305679

Synonyms

VCL; Vinculin; Metavinculin

Research area

Signal Transduction; Cardiovascular

Target protein

VCL

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 10000

Images

Western blot analysis of lysates from HeLa cells, treated with forskolin 40nM 30', using Vinculin Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using Vinculin Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Vinculin Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Vinculin Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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