Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE034 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
15000 pg/ml	2.512	2.594	2.5
	2.675
7500 pg/ml	1.242	1.194	1.1
	1.145
3750 pg/ml	0.656	0.641	0.547
	0.625
1875 pg/ml	0.482	0.46	0.366
	0.437
937.5 pg/ml	0.251	0.242	0.148
	0.232
468.75 pg/ml	0.152	0.146	0.052
	0.14
234.375 pg/ml	0.114	0.111	0.017
	0.108
0 pg/ml	0.097	0.094	0
	0.091

We measured random samples of BPE034 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	723	37.3	5.2
Intra-assay	2	20	2261	75.9	3.4
Intra-assay	3	20	9042	244	2.7
Inter-assay	1	20	721	64	8.9
Inter-assay	2	20	2249	230	10.2
Inter-assay	3	20	9075	861	9.5

> Protocol Booklet

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