Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE227 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
15000 pg/ml	2.142	2.215	2.135
	2.289
7500 pg/ml	1.22	1.256	1.176
	1.293
3750 pg/ml	0.647	0.67	0.59
	0.693
1875 pg/ml	0.368	0.365	0.285
	0.362
937.5 pg/ml	0.215	0.215	0.135
	0.216
468.75 pg/ml	0.151	0.146	0.066
	0.142
234.375 pg/ml	0.118	0.111	0.031
	0.105
0 pg/ml	0.082	0.08	0
	0.079

We measured random samples of BPE227 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	833	37.2	4.5
Intra-assay	2	20	1938	58.2	3
Intra-assay	3	20	8427	246	2.9
Inter-assay	1	20	824	65.5	7.9
Inter-assay	2	20	1944	148	7.6
Inter-assay	3	20	8436	464	5.5

> Protocol Booklet