Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Primacu > Human Aromatic-L-Amino-Acid Decarboxylase, DDC Primacu™ ELISA Kit

Human Aromatic-L-Amino-Acid Decarboxylase, DDC Primacu™ ELISA Kit

Human Aromatic-L-Amino-Acid Decarboxylase, DDC Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 101.47 pg/mL

Detection range: 234.4-15000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 101.47 pg/mL

Detection range: 234.4-15000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Aromatic-L-amino-acid decarboxylase

Code

BPE109

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

101.47 pg/mL

Detection range

234.4-15000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.

UniProt accession

MASS(Da)

53926

GeneID

1644

Synonyms

AADC

Gene names

DDC

Research area

Neuroscience,Cancer,Metabolism

Target protein

DOPA Decarboxylase

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE109 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
15000 pg/ml 2.148 2.15 2.027
2.151
7500 pg/ml 1.196 1.143 1.02
1.09
3750 pg/ml 0.655 0.627 0.504
0.598
1875 pg/ml 0.39 0.385 0.262
0.38
937.5 pg/ml 0.232 0.248 0.125
0.264
468.75 pg/ml 0.189 0.181 0.058
0.173
234.375 pg/ml 0.16 0.154 0.031
0.148
0 pg/ml 0.131 0.123 0
0.114

Precision

We measured random samples of BPE109 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 2316 153 6.6
Intra-assay 2 20 4928 221 4.5
Intra-assay 3 20 9855 288 2.9
Inter-assay 1 20 2335 169 7.3
Inter-assay 2 20 4965 253 5.1
Inter-assay 3 20 9972 454 4.6

Protocols

References

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE109

    $2320.00

  • 96T

    BPE109

    $580.00

  • 15*96T

    BPE109

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up