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Home > Product > ELISA Primacu > Human Npc Intracellular Cholesterol Transporter 2, NPC2 Primacu™ ELISA Kit

Human Npc Intracellular Cholesterol Transporter 2, NPC2 Primacu™ ELISA Kit

Human Npc Intracellular Cholesterol Transporter 2, NPC2 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 41.67 pg/mL

Detection range: 156.25-10000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 41.67 pg/mL

Detection range: 156.25-10000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

NPC intracellular cholesterol transporter 2

Code

BPE270

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

41.67 pg/mL

Detection range

156.25-10000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Niemann-Pick Type C2 (NPC2) plays an important role in the regulation of intracellular cholesterol homeostasis via direct binding with free cholesterol. NPC2 is an intralysosomal protein that binds cholesterol in vitro. NPC2 is a small lysosomal glycoprotein that binds cholesterol with submicromolar affinity. Deficiency in NPC2 is the cause of Niemann-Pick type C2 disease, a fatal neurovisceral disorder characterized by the accumulation of cholesterol in lysosomes. Niemann-Pick disease, type C2 (NPC2) protein is one of the most abundant components of the epididymal fluid and contains a functional cholesterol-binding site that can transfer cholesterol between membranes, it has been suggested for years that NPC2 could be involved in the regulation of cholesterol levels in spermatozoa during epididymal maturation.

UniProt accession

MASS(Da)

16570

GeneID

10577

Synonyms

HE1

Gene names

NPC2

Research area

Cardiovascular,Neuroscience,Metabolism,Signal transduction

Target protein

NPC2

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE270 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
10000 pg/ml 2.718 2.59 2.464
2.463
5000 pg/ml 1.426 1.449 1.323
1.473
2500 pg/ml 0.838 0.794 0.668
0.751
1250 pg/ml 0.445 0.468 0.342
0.491
625 pg/ml 0.28 0.298 0.172
0.317
312.5 pg/ml 0.203 0.214 0.088
0.225
156.25 pg/ml 0.163 0.17 0.044
0.178
0 pg/ml 0.132 0.126 0
0.12

Precision

We measured random samples of BPE270 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 340 23.5 6.9
Intra-assay 2 20 1701 90 5.3
Intra-assay 3 20 6074 179 2.9
Inter-assay 1 20 338 27.2 8.1
Inter-assay 2 20 1712 119 7
Inter-assay 3 20 6030 359 6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE270

    $2320.00

  • 96T

    BPE270

    $580.00

  • 15*96T

    BPE270

    $6525.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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