Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE273 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
10000 pg/ml	2.176	2.185	2.052
	2.194
5000 pg/ml	1.159	1.21	1.077
	1.261
2500 pg/ml	0.754	0.728	0.595
	0.702
1250 pg/ml	0.395	0.417	0.284
	0.439
625 pg/ml	0.278	0.289	0.156
	0.301
312.5 pg/ml	0.236	0.24	0.107
	0.245
156.25 pg/ml	0.184	0.182	0.049
	0.181
0 pg/ml	0.142	0.133	0
	0.124

We measured random samples of BPE273 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	804	80.2	10
Intra-assay	2	20	1711	58.4	3.4
Intra-assay	3	20	6109	204	3.3
Inter-assay	1	20	797	75.1	9.4
Inter-assay	2	20	1691	132	7.8
Inter-assay	3	20	6150	497	8.1

> Protocol Booklet