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Home > Product > ELISA Primacu > Human Coagulation Factor Ix, F9 Primacu™ ELISA Kit

Human Coagulation Factor Ix, F9 Primacu™ ELISA Kit

Human Coagulation Factor Ix, F9 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 44.93 pg/mL

Detection range: 140.63-9000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 44.93 pg/mL

Detection range: 140.63-9000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Coagulation factor IX

Code

BPE198

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

44.93 pg/mL

Detection range

140.63-9000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one?peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B ( HEMB ) which also known as Christmas disease.

UniProt accession

MASS(Da)

51778

GeneID

2158

Gene names

F9

Research area

Cardiovascular,Immunology

Target protein

Factor IX

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE198 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
9000 pg/ml 2.253 2.298 2.195
2.342
4500 pg/ml 1.227 1.216 1.113
1.204
2250 pg/ml 0.667 0.661 0.558
0.655
1125 pg/ml 0.394 0.372 0.269
0.35
562.5 pg/ml 0.23 0.235 0.132
0.239
281.25 pg/ml 0.175 0.18 0.077
0.184
140.625 pg/ml 0.131 0.134 0.031
0.136
0 pg/ml 0.108 0.103 0
0.098

Precision

We measured random samples of BPE198 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 551 23.6 4.3
Intra-assay 2 20 1837 50.8 2.8
Intra-assay 3 20 6335 142 2.2
Inter-assay 1 20 548 43.3 7.9
Inter-assay 2 20 1827 113 6.2
Inter-assay 3 20 6268 362 5.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE198

    $2040.00

  • 96T

    BPE198

    $480.00

  • 15*96T

    BPE198

    $5400.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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