Full product name

Code

Assay type

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Sensitivity

Detection range

Sample type

Species

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Assay time

Background

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE346 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8000 pg/ml	1.9	1.884	1.757
	1.868
4000 pg/ml	1.197	1.216	1.089
	1.236
2000 pg/ml	0.728	0.746	0.619
	0.764
1000 pg/ml	0.465	0.463	0.336
	0.462
500 pg/ml	0.288	0.303	0.176
	0.319
250 pg/ml	0.197	0.21	0.083
	0.224
125 pg/ml	0.172	0.166	0.039
	0.16
0 pg/ml	0.127	0.127	0
	0.128

We measured random samples of BPE346 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	1152	65.4	5.7
Intra-assay	2	20	2743	131	4.8
Intra-assay	3	20	5714	289	5.1
Inter-assay	1	20	1150	60.7	5.3
Inter-assay	2	20	2752	117	4.2
Inter-assay	3	20	5706	409	7.2

> Protocol Booklet

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