Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE123 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
5000 pg/ml	2.68	2.599	2.508
	2.518
2500 pg/ml	1.525	1.5	1.409
	1.475
1250 pg/ml	0.83	0.832	0.741
	0.834
625 pg/ml	0.432	0.447	0.356
	0.461
312.5 pg/ml	0.28	0.267	0.176
	0.254
156.25 pg/ml	0.181	0.177	0.086
	0.173
78.125 pg/ml	0.143	0.134	0.043
	0.125
0 pg/ml	0.094	0.091	0
	0.088

We measured random samples of BPE123 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	266	14.5	5.5
Intra-assay	2	20	1024	47.8	4.7
Intra-assay	3	20	2925	63.4	2.2
Inter-assay	1	20	265	20.5	7.7
Inter-assay	2	20	1015	79	7.8
Inter-assay	3	20	2941	188	6.4

> Protocol Booklet