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Home > Product > ELISA Primacu > Human IFNAR1 Primacu™ ELISA Kit

Human IFNAR1 Primacu™ ELISA Kit

Human IFNAR1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 22.52 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 22.52 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interferon alpha/beta receptor 1

Code

BPE269

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

22.52 pg/mL

Detection range

78.13-5000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Interferon-alpha/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulate Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due, at least in part, to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1, in which Tyr466 is changed to phenylalanine, can act in a dominant-negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.

UniProt accession

MASS(Da)

63525

GeneID

3454

Synonyms

IFNAR

Gene names

IFNAR1

Research area

Cancer,Immunology

Target protein

IFNAR1

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE269 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
5000 pg/ml 2.258 2.332 2.219
2.405
2500 pg/ml 1.281 1.239 1.126
1.196
1250 pg/ml 0.707 0.678 0.565
0.648
625 pg/ml 0.4 0.408 0.295
0.415
312.5 pg/ml 0.274 0.259 0.146
0.243
156.25 pg/ml 0.193 0.189 0.076
0.184
78.125 pg/ml 0.147 0.151 0.038
0.154
0 pg/ml 0.119 0.113 0
0.106

Precision

We measured random samples of BPE269 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 307 15.2 5
Intra-assay 2 20 652 31.7 4.9
Intra-assay 3 20 2609 129 5
Inter-assay 1 20 308 25.8 8.4
Inter-assay 2 20 649 46 7.1
Inter-assay 3 20 2597 148 5.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE269

    $2320.00

  • 96T

    BPE269

    $580.00

  • 15*96T

    BPE269

    $6525.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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