Full product name

Code

Assay type

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Detection range

Sample type

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Assay time

Background

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE344 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
5000 pg/ml	2.462	2.367	2.252
	2.272
2500 pg/ml	1.187	1.154	1.039
	1.121
1250 pg/ml	0.549	0.556	0.441
	0.562
625 pg/ml	0.294	0.305	0.19
	0.315
312.5 pg/ml	0.197	0.187	0.072
	0.177
156.25 pg/ml	0.157	0.147	0.032
	0.137
78.125 pg/ml	0.134	0.129	0.014
	0.124
0 pg/ml	0.107	0.115	0
	0.123

We measured random samples of BPE344 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	469	18.2	3.9
Intra-assay	2	20	1234	41.5	3.4
Intra-assay	3	20	3010	143	4.8
Inter-assay	1	20	474	38.8	8.2
Inter-assay	2	20	1223	80.6	6.6
Inter-assay	3	20	2991	176	5.9

> Protocol Booklet

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