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Home > Product > ELISA Primacu > Human Mhc Class I Polypeptide-Related Sequence B, MICB Primacu™ ELISA Kit

Human Mhc Class I Polypeptide-Related Sequence B, MICB Primacu™ ELISA Kit

Human Mhc Class I Polypeptide-Related Sequence B, MICB Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 24.49 pg/mL

Detection range: 78.12-5000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 24.49 pg/mL

Detection range: 78.12-5000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

MHC class I polypeptide-related sequence B

Code

BPE381

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

24.49 pg/mL

Detection range

78.12-5000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without the capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B is minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.

UniProt accession

MASS(Da)

42575

GeneID

4277

Synonyms

PERB11.2

Gene names

MICB

Research area

Immunology

Target protein

MICB

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE381 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
5000 pg/ml 2.213 2.321 2.233
2.429
2500 pg/ml 1.269 1.274 1.186
1.279
1250 pg/ml 0.717 0.695 0.607
0.674
625 pg/ml 0.394 0.376 0.288
0.358
312.5 pg/ml 0.263 0.246 0.158
0.229
156.25 pg/ml 0.17 0.169 0.081
0.168
78.125 pg/ml 0.136 0.128 0.04
0.12
0 pg/ml 0.092 0.088 0
0.084

Precision

We measured random samples of BPE381 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 385 21.1 5.5
Intra-assay 2 20 802 26.2 3.3
Intra-assay 3 20 2767 62.8 2.3
Inter-assay 1 20 389 21.5 5.5
Inter-assay 2 20 792 42.6 5.4
Inter-assay 3 20 2790 137 4.9

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE381

    $2040.00

  • 96T

    BPE381

    $480.00

  • 15*96T

    BPE381

    $5400.00

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Lead time: Within two weeks

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