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Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE002 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000 pg/ml	2.475	2.442	2.317
	2.408
2000 pg/ml	1.302	1.307	1.182
	1.312
1000 pg/ml	0.743	0.697	0.572
	0.65
500 pg/ml	0.431	0.413	0.288
	0.395
250 pg/ml	0.27	0.263	0.138
	0.256
125 pg/ml	0.197	0.191	0.066
	0.185
62.5 pg/ml	0.162	0.156	0.031
	0.149
0 pg/ml	0.125	0.125	0
	0.124

We measured random samples of BPE002 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	216	12.8	5.9
Intra-assay	2	20	983	36.2	3.7
Intra-assay	3	20	2658	116	4.4
Inter-assay	1	20	216	20.3	9.4
Inter-assay	2	20	979	48.1	4.9
Inter-assay	3	20	2645	125	4.7

> Protocol Booklet

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