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Home > Product > ELISA Primacu > Human Lymphokine-Activated Killer T-Cell-Originated Protein Kinase, PB Primacu™ ELISA Kit

Human Lymphokine-Activated Killer T-Cell-Originated Protein Kinase, PB Primacu™ ELISA Kit

Human Lymphokine-Activated Killer T-Cell-Originated Protein Kinase, PB Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 21.19 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 21.19 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Lymphokine-activated killer T-cell-originated protein kinase

Code

BPE126

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

21.19 pg/mL

Detection range

62.5-4000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

PDZ binding kinase (PBK), also known as TOPK (T-LAK cell-originated protein kinase), is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family, and has all the characteristic protein kinase subdomains and a C-terminal PDZ-binding T/SXV motif. PBK is expressed in the testis restrictedly expressed in outer cell layer of seminiferous tubules, as well as placenta. PBK may be enrolled in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. This mitotic kinase phosphorylates MAP kinase p38 and seems to be active in mitosis. When phosphorylated, PBK forms a protein-protein interaction with tumor suppressor p53 (TP53), leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. The expression level of PBK is thus upregulated in a variety of neoplasms including hematological malignancies.

UniProt accession

MASS(Da)

36085

GeneID

55872

Synonyms

TOPK

Gene names

PBK

Research area

Cancer,Cell biology,Epigenetics and nuclear signaling,Signal transduction

Target protein

PBK

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE126 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000 pg/ml 1.46 1.492 1.369
1.523
2000 pg/ml 1.014 1.012 0.889
1.009
1000 pg/ml 0.61 0.646 0.523
0.681
500 pg/ml 0.443 0.417 0.294
0.39
250 pg/ml 0.288 0.277 0.154
0.266
125 pg/ml 0.197 0.202 0.079
0.206
62.5 pg/ml 0.151 0.162 0.039
0.172
0 pg/ml 0.126 0.123 0
0.12

Precision

We measured random samples of BPE126 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 274 10.6 3.9
Intra-assay 2 20 740 16.8 2.3
Intra-assay 3 20 2644 78.1 3
Inter-assay 1 20 272 22 8.1
Inter-assay 2 20 735 59.3 8.1
Inter-assay 3 20 2618 161 6.2

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE126

    $2320.00

  • 96T

    BPE126

    $580.00

  • 15*96T

    BPE126

    $6525.00

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Lead time: Within two weeks

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