Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE271 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000 pg/ml	2.356	2.436	2.339
	2.516
2000 pg/ml	1.42	1.408	1.311
	1.396
1000 pg/ml	0.791	0.743	0.646
	0.694
500 pg/ml	0.409	0.406	0.309
	0.403
250 pg/ml	0.247	0.252	0.155
	0.257
125 pg/ml	0.167	0.171	0.074
	0.175
62.5 pg/ml	0.157	0.147	0.05
	0.137
0 pg/ml	0.091	0.097	0
	0.103

We measured random samples of BPE271 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	428	42.7	10
Intra-assay	2	20	1259	42.4	3.4
Intra-assay	3	20	2928	97.3	3.3
Inter-assay	1	20	426	40.4	9.5
Inter-assay	2	20	1269	98.7	7.8
Inter-assay	3	20	2905	229	7.9

> Protocol Booklet