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Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE280 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000 pg/ml	2.247	2.226	2.133
	2.205
2000 pg/ml	1.214	1.246	1.153
	1.278
1000 pg/ml	0.707	0.678	0.585
	0.65
500 pg/ml	0.375	0.364	0.271
	0.354
250 pg/ml	0.257	0.249	0.156
	0.242
125 pg/ml	0.169	0.168	0.075
	0.167
62.5 pg/ml	0.126	0.131	0.038
	0.136
0 pg/ml	0.1	0.093	0
	0.087

We measured random samples of BPE280 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	388	34.8	9
Intra-assay	2	20	1251	46.6	3.7
Intra-assay	3	20	2908	140	4.8
Inter-assay	1	20	383	12.5	3.3
Inter-assay	2	20	1239	108	8.8
Inter-assay	3	20	2892	130	4.5

> Protocol Booklet

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