Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE315 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000 pg/ml	2.417	2.302	2.215
	2.188
2000 pg/ml	1.407	1.363	1.276
	1.32
1000 pg/ml	0.733	0.772	0.685
	0.811
500 pg/ml	0.403	0.421	0.334
	0.44
250 pg/ml	0.245	0.252	0.165
	0.259
125 pg/ml	0.174	0.165	0.078
	0.157
62.5 pg/ml	0.123	0.12	0.033
	0.118
0 pg/ml	0.091	0.087	0
	0.083

We measured random samples of BPE315 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	174	11.9	6.8
Intra-assay	2	20	623	28.6	4.6
Intra-assay	3	20	2708	105	3.9
Inter-assay	1	20	173	15.5	8.9
Inter-assay	2	20	616	33.2	5.4
Inter-assay	3	20	2739	191	7

> Protocol Booklet