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Home > Product > ELISA Primacu > Human Granulocyte Colony-Stimulating Factor Receptor, CSF3R Primacu™ ELISA Kit

Human Granulocyte Colony-Stimulating Factor Receptor, CSF3R Primacu™ ELISA Kit

Human Granulocyte Colony-Stimulating Factor Receptor, CSF3R Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 11.34 pg/mL

Detection range: 50-3200 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 11.34 pg/mL

Detection range: 50-3200 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Granulocyte colony-stimulating factor receptor

Code

BPE048

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

11.34 pg/mL

Detection range

50-3200 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.

UniProt accession

MASS(Da)

92156

GeneID

1441

Synonyms

GCSFR

Gene names

CSF3R

Research area

Cancer,Immunology,Stem cells

Target protein

CSF3R

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE048 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200 pg/ml 2.387 2.31 2.207
2.233
1600 pg/ml 1.335 1.269 1.166
1.204
800 pg/ml 0.749 0.702 0.599
0.656
400 pg/ml 0.38 0.381 0.278
0.383
200 pg/ml 0.238 0.24 0.137
0.243
100 pg/ml 0.18 0.168 0.065
0.157
50 pg/ml 0.139 0.134 0.031
0.13
0 pg/ml 0.106 0.103 0
0.101

Precision

We measured random samples of BPE048 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 333 22.6 6.8
Intra-assay 2 20 679 24.1 3.5
Intra-assay 3 20 1997 96.8 4.8
Inter-assay 1 20 335 20.5 6.1
Inter-assay 2 20 671 33.6 5
Inter-assay 3 20 1990 110 5.5

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE048

    $2040.00

  • 96T

    BPE048

    $480.00

  • 15*96T

    BPE048

    $5400.00

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Lead time: Within two weeks

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