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Home > Product > ELISA Primacu > Mouse T-Cell Surface Glycoprotein Cd8 Alpha Chain, Cd8a Primacu™ ELISA Kit

Mouse T-Cell Surface Glycoprotein Cd8 Alpha Chain, Cd8a Primacu™ ELISA Kit

Mouse T-Cell Surface Glycoprotein Cd8 Alpha Chain, Cd8a Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 19.53 pg/mL

Detection range: 46.88-3000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 19.53 pg/mL

Detection range: 46.88-3000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

T-cell surface glycoprotein CD8 alpha chain

Code

BPE313

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

19.53 pg/mL

Detection range

46.88-3000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8°C

Assay time

3.5h

Background

T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

UniProt accession

MASS(Da)

27456

GeneID

12525

Synonyms

Lyt-2;Lyt2

Gene names

Cd8a

Research area

Cancer,Immunology,Stem cells

Target protein

CD8 alpha

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE313 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3000 pg/ml 1.514 1.541 1.459
1.568
1500 pg/ml 0.894 0.904 0.822
0.914
750 pg/ml 0.569 0.554 0.472
0.539
375 pg/ml 0.31 0.323 0.241
0.336
187.5 pg/ml 0.2 0.204 0.122
0.208
93.75 pg/ml 0.15 0.147 0.065
0.144
46.875 pg/ml 0.114 0.112 0.03
0.11
0 pg/ml 0.086 0.082 0
0.079

Precision

We measured random samples of BPE313 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 274 11.7 4.3
Intra-assay 2 20 548 17.9 3.3
Intra-assay 3 20 1890 74.5 3.9
Inter-assay 1 20 272 28.9 10.6
Inter-assay 2 20 551 37.1 6.7
Inter-assay 3 20 1903 105 5.5

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE313

    $2320.00

  • 96T

    BPE313

    $580.00

  • 15*96T

    BPE313

    $6525.00

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Lead time: Within two weeks

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