Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE329 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3000 pg/ml	2.333	2.287	2.198
	2.242
1500 pg/ml	1.259	1.209	1.12
	1.159
750 pg/ml	0.649	0.663	0.574
	0.677
375 pg/ml	0.389	0.372	0.283
	0.355
187.5 pg/ml	0.25	0.235	0.146
	0.22
93.75 pg/ml	0.167	0.162	0.073
	0.157
46.875 pg/ml	0.129	0.124	0.035
	0.119
0 pg/ml	0.085	0.089	0
	0.093

We measured random samples of BPE329 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	141	6.3	4.5
Intra-assay	2	20	542	10.4	1.9
Intra-assay	3	20	1869	44.4	2.4
Inter-assay	1	20	140	9.5	6.8
Inter-assay	2	20	539	19.4	3.6
Inter-assay	3	20	1850	75.4	4.1

> Protocol Booklet