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Home > Product > ELISA Primacu > Human Interleukin-6 Receptor Subunit Alpha, IL6R Primacu™ ELISA Kit

Human Interleukin-6 Receptor Subunit Alpha, IL6R Primacu™ ELISA Kit

Human Interleukin-6 Receptor Subunit Alpha, IL6R Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 8.48 pg/mL

Detection range: 46.88-3000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 8.48 pg/mL

Detection range: 46.88-3000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin-6 receptor subunit alpha

Code

BPE378

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

8.48 pg/mL

Detection range

46.88-3000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (SIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a SIL-6R protein can mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signaling. Through this so-called 'trans-signaling' mechanism, IL-6 can stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and SIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.

UniProt accession

MASS(Da)

51548

GeneID

3570

Gene names

IL6R

Research area

Immunology,Neuroscience,Stem cells

Target protein

IL-6R

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE378 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3000 pg/ml 2.845 2.873 2.763
2.901
1500 pg/ml 1.433 1.457 1.347
1.481
750 pg/ml 0.739 0.747 0.637
0.754
375 pg/ml 0.397 0.376 0.266
0.355
187.5 pg/ml 0.232 0.231 0.121
0.229
93.75 pg/ml 0.16 0.157 0.047
0.153
46.875 pg/ml 0.125 0.124 0.014
0.122
0 pg/ml 0.104 0.11 0
0.116

Precision

We measured random samples of BPE378 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 405 17.2 4.2
Intra-assay 2 20 943 32 3.4
Intra-assay 3 20 2143 102 4.7
Inter-assay 1 20 403 40.3 10
Inter-assay 2 20 946 64.9 6.9
Inter-assay 3 20 2147 61.8 2.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE378

    $2040.00

  • 96T

    BPE378

    $480.00

  • 15*96T

    BPE378

    $5400.00

Add to Cart

Lead time: Within two weeks

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