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Home > Product > ELISA Primacu > Human Cartilage Oligomeric Matrix Protein, COMP Primacu™ ELISA Kit

Human Cartilage Oligomeric Matrix Protein, COMP Primacu™ ELISA Kit

Human Cartilage Oligomeric Matrix Protein, COMP Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 17.92 pg/mL

Detection range: 39.06-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 17.92 pg/mL

Detection range: 39.06-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cartilage oligomeric matrix protein

Code

BPE037

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

17.92 pg/mL

Detection range

39.06-2500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Cartilage Oligomeric Matrix Protein (COMP), also referred to as Thrombospondin-5, is a non-collagenous extracellular matrix (ECM) protein and belongs to the subgroup B of the thrombospondin protein family. This protein is expressed primarily in cartilage, ligament, and tendon, and binds to other ECM proteins such as collagen I, II and IX with high affinities depending on the divalent cations Zn2+ or Ni2+. COMP is a secreted glycoprotein that is important for growth plate organization and function. It is suggested to play a role in cell growth and development, and recent studies have revealed the possible mechanism that it protects cells against death by elevating members of the IAP (inhibitor of apoptosis protein) family of survival proteins. Mutations in COMP cause two skeletal dysplasias, pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (EDM1), and up-regulated expression of COMP are observed in rheumatoid arthritis and certain carcinomas.

UniProt accession

MASS(Da)

82860

GeneID

1311

Gene names

COMP

Research area

Cell biology,Signal transduction,Stem cells

Target protein

COMP

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE037 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2500 pg/ml 2.64 2.664 2.537
2.689
1250 pg/ml 1.592 1.566 1.439
1.54
625 pg/ml 0.912 0.896 0.769
0.88
312.5 pg/ml 0.503 0.498 0.3705
0.492
156.25 pg/ml 0.294 0.291 0.164
0.288
78.125 pg/ml 0.209 0.199 0.072
0.19
39.0625 pg/ml 0.154 0.153 0.026
0.152
0 pg/ml 0.133 0.127 0
0.121

Precision

We measured random samples of BPE037 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 281 22.7 8.1
Intra-assay 2 20 758 39.2 5.2
Intra-assay 3 20 1764 92.8 5.3
Inter-assay 1 20 278 19.9 7.1
Inter-assay 2 20 754 47.3 6.3
Inter-assay 3 20 1756 85.6 4.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE037

    $2040.00

  • 96T

    BPE037

    $480.00

  • 15*96T

    BPE037

    $5400.00

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Lead time: Within two weeks

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