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Home > Product > ELISA Primacu > Human Kallikrein-13, KLK13 Primacu™ ELISA Kit

Human Kallikrein-13, KLK13 Primacu™ ELISA Kit

Human Kallikrein-13, KLK13 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 10.79 pg/mL

Detection range: 39.06-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 10.79 pg/mL

Detection range: 39.06-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Kallikrein-13

Code

BPE042

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

10.79 pg/mL

Detection range

39.06-2500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Tissue kallikrein 13 (hK13), also known as KLK-L4 (kallikrein-like gene 4), is a member of the human tissue kallikrein family of serine proteases having diverse physiological functions in many tissues. The KLK13 gene resides on chromosome 19q13.3-4 along with other 14 members in a gene cluster and shares a high degree of homology. KLK13 is a trypsin-like, secreted serine protease expressed specifically in the testicular tissue including prostate, salivary gland, breast, and testis. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and may play a role in metastasis. KLK13 may be involved in the pathogenesis and/or progression of breast and ovary cancers and is regarded as a novel cancer biomarker. Besides, KLK13 interacts and forms complexes with several serum protease inhibitors, such as alpha2-macroglobulin, and its expression is regulated by steroid hormones.

UniProt accession

MASS(Da)

30570

GeneID

26085

Synonyms

KLKL4

Gene names

KLK13

Research area

Cancer,Cell biology,Signal transduction

Target protein

Kallikrein 13

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE042 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2500 pg/ml 1.836 1.795 1.686
1.755
1250 pg/ml 1.061 1.043 0.934
1.026
625 pg/ml 0.647 0.673 0.564
0.7
312.5 pg/ml 0.401 0.386 0.277
0.372
156.25 pg/ml 0.224 0.237 0.128
0.251
78.125 pg/ml 0.163 0.163 0.054
0.164
39.0625 pg/ml 0.131 0.14 0.031
0.15
0 pg/ml 0.103 0.109 0
0.116

Precision

We measured random samples of BPE042 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 450 38.6 8.6
Intra-assay 2 20 938 43.9 4.7
Intra-assay 3 20 1997 99.9 5
Inter-assay 1 20 448 39.5 8.8
Inter-assay 2 20 929 56.3 6.1
Inter-assay 3 20 1979 134 6.8

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE042

    $2320.00

  • 96T

    BPE042

    $580.00

  • 15*96T

    BPE042

    $6525.00

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Lead time: Within two weeks

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