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Home > Product > ELISA Primacu > Human Extracellular Matrix Protein 1, ECM1 Primacu™ ELISA Kit

Human Extracellular Matrix Protein 1, ECM1 Primacu™ ELISA Kit

Human Extracellular Matrix Protein 1, ECM1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 7.47 pg/mL

Detection range: 39.06-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 7.47 pg/mL

Detection range: 39.06-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Extracellular matrix protein 1

Code

BPE072

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

7.47 pg/mL

Detection range

39.06-2500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Extracellular matrix protein 1 (ECM1) is a secreted glycoprotein and playing a pivotal role in endochondral bone formation, angiogenesis, and tumour biology. Three splice variants have been identified: ECM1a (540 aa) is most widely expressed, with highest expression in the placenta and heart; ECM1b (415 aa) is differentiation-dependent expressed and found only in tonsil and associated with suprabasal keratinocytes; ECM1c (559 aa) accounts for approximately 15% of skin ECM1. Although ECM1 is not tumor specific, is significantly elevated in many malignant epithelial tumors and is suggested as a possible trigger for angiogenesis, tumor progression and malignancies. It also has been shown to regulate endochondral bone formation, skeletal development and tissue remodeling.

UniProt accession

MASS(Da)

60674

GeneID

1893

Gene names

ECM1

Research area

Immunology,Tags & cell markers

Target protein

ECM1

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE072 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2500 pg/ml 2.361 2.33 2.217
2.299
1250 pg/ml 1.283 1.309 1.196
1.334
625 pg/ml 0.752 0.715 0.602
0.677
312.5 pg/ml 0.437 0.424 0.311
0.41
156.25 pg/ml 0.247 0.261 0.148
0.274
78.125 pg/ml 0.19 0.187 0.074
0.184
39.0625 pg/ml 0.153 0.15 0.037
0.146
0 pg/ml 0.12 0.113 0
0.106

Precision

We measured random samples of BPE072 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 317 23 7.2
Intra-assay 2 20 774 53.8 6.9
Intra-assay 3 20 1799 81.2 4.5
Inter-assay 1 20 319 32.2 10.1
Inter-assay 2 20 765 72.8 9.5
Inter-assay 3 20 1817 139 7.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE072

    $2320.00

  • 96T

    BPE072

    $580.00

  • 15*96T

    BPE072

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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