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Home > Product > ELISA Primacu > Human Asialoglycoprotein Receptor 1, ASGR1 Primacu™ ELISA Kit

Human Asialoglycoprotein Receptor 1, ASGR1 Primacu™ ELISA Kit

Human Asialoglycoprotein Receptor 1, ASGR1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 15.32 pg/mL

Detection range: 39.06-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 15.32 pg/mL

Detection range: 39.06-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Asialoglycoprotein receptor 1

Code

BPE130

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

15.32 pg/mL

Detection range

39.06-2500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development.

UniProt accession

MASS(Da)

33186

GeneID

432

Synonyms

CLEC4H1

Gene names

ASGR1

Research area

Signal transduction

Target protein

ASGR1

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE130 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2500 pg/ml 2.48 2.523 2.438
2.566
1250 pg/ml 1.373 1.418 1.333
1.462
625 pg/ml 0.729 0.748 0.663
0.767
312.5 pg/ml 0.418 0.425 0.34
0.431
156.25 pg/ml 0.233 0.244 0.159
0.255
78.125 pg/ml 0.154 0.16 0.075
0.166
39.0625 pg/ml 0.115 0.121 0.036
0.126
0 pg/ml 0.086 0.085 0
0.084

Precision

We measured random samples of BPE130 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 256 10.8 4.2
Intra-assay 2 20 801 29.7 3.7
Intra-assay 3 20 1864 69.8 3.7
Inter-assay 1 20 255 33.2 13
Inter-assay 2 20 804 77.7 9.7
Inter-assay 3 20 1886 163 8.6

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE130

    $2320.00

  • 96T

    BPE130

    $580.00

  • 15*96T

    BPE130

    $6525.00

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Lead time: Within two weeks

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