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Home > Product > ELISA Primacu > Human CD38 Primacu™ ELISA Kit

Human CD38 Primacu™ ELISA Kit

Human CD38 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 14.91 pg/mL

Detection range: 39.06-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 14.91 pg/mL

Detection range: 39.06-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1

Code

BPE136

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

14.91 pg/mL

Detection range

39.06-2500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 38 (CD38), also known as ADP-ribosyl cyclase, is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B and natural killer cells. It shares several characteristics with ADP-ribosyl cyclase 2 CD157. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. It also functions in cell adhesion, signal transduction and calcium signaling. CD38 has been used as a prognostic marker in leukemia. It can also be used to identify plasma cells.

UniProt accession

MASS(Da)

34328

GeneID

952

Gene names

CD38

Research area

Cancer,Immunology,Metabolism,Microbiology,Stem cells

Target protein

CD38

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE136 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6000 pg/ml 2.465 2.579 2.475
2.694
3000 pg/ml 1.499 1.45 1.346
1.402
1500 pg/ml 0.79 0.814 0.71
0.838
750 pg/ml 0.499 0.483 0.379
0.467
375 pg/ml 0.315 0.303 0.199
0.292
187.5 pg/ml 0.193 0.201 0.097
0.209
93.75 pg/ml 0.158 0.149 0.045
0.14
0 pg/ml 0.106 0.104 0
0.102

Precision

We measured random samples of BPE136 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 349 16.2 4.6
Intra-assay 2 20 759 15.4 2
Intra-assay 3 20 1651 40.3 2.4
Inter-assay 1 20 352 24.2 6.9
Inter-assay 2 20 756 28.4 3.8
Inter-assay 3 20 1639 66.9 4.1

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE136

    $2320.00

  • 96T

    BPE136

    $580.00

  • 15*96T

    BPE136

    $6525.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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