Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE370 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2500 pg/ml	2.509	2.574	2.453
	2.638
1250 pg/ml	1.271	1.235	1.114
	1.198
625 pg/ml	0.646	0.612	0.491
	0.578
312.5 pg/ml	0.325	0.319	0.198
	0.313
156.25 pg/ml	0.231	0.218	0.097
	0.204
78.125 pg/ml	0.173	0.17	0.049
	0.166
39.0625 pg/ml	0.156	0.148	0.027
	0.139
0 pg/ml	0.128	0.121	0
	0.113

We measured random samples of BPE370 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	161	14.9	9.3
Intra-assay	2	20	460	19.4	4.2
Intra-assay	3	20	1314	56.8	4.3
Inter-assay	1	20	162	10	6.2
Inter-assay	2	20	457	20.9	4.6
Inter-assay	3	20	1312	62.7	4.8

> Protocol Booklet

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