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Home > Product > ELISA Primacu > Human Dickkopf-Related Protein 3, DKK3 Primacu™ ELISA Kit

Human Dickkopf-Related Protein 3, DKK3 Primacu™ ELISA Kit

Human Dickkopf-Related Protein 3, DKK3 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 12.33 pg/mL

Detection range: 39.1-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 12.33 pg/mL

Detection range: 39.1-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dickkopf-related protein 3

Code

BPE372

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

12.33 pg/mL

Detection range

39.1-2500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.

UniProt accession

MASS(Da)

38390

GeneID

27122

Synonyms

REIC

Gene names

DKK3

Research area

Stem cells,Signal transduction

Target protein

DKK3

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE372 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2500 pg/ml 2.393 2.436 2.312
2.48
1250 pg/ml 1.381 1.362 1.238
1.344
625 pg/ml 0.751 0.786 0.662
0.822
312.5 pg/ml 0.45 0.457 0.333
0.465
156.25 pg/ml 0.313 0.294 0.17
0.276
78.125 pg/ml 0.214 0.221 0.097
0.229
39.0625 pg/ml 0.191 0.186 0.062
0.182
0 pg/ml 0.126 0.124 0
0.122

Precision

We measured random samples of BPE372 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 150 6.9 4.6
Intra-assay 2 20 384 20.1 5.2
Intra-assay 3 20 1669 104 6.2
Inter-assay 1 20 151 9.4 6.2
Inter-assay 2 20 382 19.9 5.2
Inter-assay 3 20 1657 82.1 5

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE372

    $2040.00

  • 96T

    BPE372

    $480.00

  • 15*96T

    BPE372

    $5400.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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