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Home > Product > ELISA Primacu > Human Interleukin-13 Receptor Subunit Alpha-1, IL13RA1 Primacu™ ELISA Kit

Human Interleukin-13 Receptor Subunit Alpha-1, IL13RA1 Primacu™ ELISA Kit

Human Interleukin-13 Receptor Subunit Alpha-1, IL13RA1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 6.57 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 6.57 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin-13 receptor subunit alpha-1

Code

BPE158

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

6.57 pg/mL

Detection range

31.25-2000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Interleukin 13 receptor, alpha 1, also known as IL13RA1/IL-13RA1 and CD213A1 (cluster of differentiation 213A1), is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. IL13RA1/IL-13RA1 serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2 and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3, and STAT6 induced by IL13 and IL4. IL13RA1/IL-13RA1 binds with low affinity to interleukin-13 (IL13). This subunit together with IL4RA can form a functional receptor for IL13. IL13RA1/IL-13RA1 also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.

UniProt accession

MASS(Da)

48760

GeneID

3597

Synonyms

IL13R;IL13RA

Gene names

IL13RA1

Research area

Immunology,Signal transduction

Target protein

IL13RA1

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE158 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000 pg/ml 1.706 1.616 1.519
1.525
1000 pg/ml 0.934 0.989 0.892
1.043
500 pg/ml 0.579 0.599 0.502
0.618
250 pg/ml 0.342 0.347 0.25
0.351
125 pg/ml 0.214 0.223 0.126
0.231
62.5 pg/ml 0.163 0.158 0.061
0.153
31.25 pg/ml 0.132 0.125 0.028
0.117
0 pg/ml 0.098 0.097 0
0.095

Precision

We measured random samples of BPE158 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 189 18.9 10
Intra-assay 2 20 572 19.3 3.4
Intra-assay 3 20 1301 45.2 3.5
Inter-assay 1 20 188 18 9.6
Inter-assay 2 20 567 44 7.8
Inter-assay 3 20 1309 105 8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE158

    $2040.00

  • 96T

    BPE158

    $480.00

  • 15*96T

    BPE158

    $5400.00

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Lead time: Within two weeks

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