Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE252 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000 pg/ml	2.527	2.414	2.307
	2.301
1000 pg/ml	1.17	1.188	1.081
	1.206
500 pg/ml	0.673	0.663	0.556
	0.653
250 pg/ml	0.387	0.379	0.272
	0.371
125 pg/ml	0.246	0.237	0.13
	0.227
62.5 pg/ml	0.171	0.166	0.059
	0.16
31.25 pg/ml	0.133	0.14	0.033
	0.146
0 pg/ml	0.11	0.107	0
	0.104

We measured random samples of BPE252 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	266	14	5.3
Intra-assay	2	20	592	19.3	3.3
Intra-assay	3	20	1409	78.5	5.6
Inter-assay	1	20	263	8.9	3.4
Inter-assay	2	20	587	48.7	8.3
Inter-assay	3	20	1401	46.9	3.3

> Protocol Booklet