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Home > Product > ELISA Primacu > Human Interleukin-19, IL19 Primacu™ ELISA Kit

Human Interleukin-19, IL19 Primacu™ ELISA Kit

Human Interleukin-19, IL19 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 5.92 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 5.92 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin-19

Code

BPE263

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

5.92 pg/mL

Detection range

31.25-2000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

The molecular features at the IL19 locus may modestly alter the establishment of HIV-1 infection. Interleukin (IL) 19, IL-20, and IL-24 belong to the IL-10 cytokine family and have been identified to play a role in the regulation of epidermal functions and inflammation. The expression of IL19 in biopsies of patients with active ulcerative colitis was increased compared with patients with quiescent ulcerative colitis and that colitis was attenuated in IL-19-deficient mice. The disruption of the epithelial barrier with dextran sodium sulfate leads to increased IL-19 expression. Attenuated colitis in IL-19-deficient animals was associated with reduced numbers of IL-6-producing macrophages in the inflamed colonic lamina propria. Microbial-driven expression of IL-19 by intestinal macrophages may contribute to the pathogenesis of inflammatory bowel disease.

UniProt accession

MASS(Da)

20452

GeneID

29949

Synonyms

ZMDA1

Gene names

IL19

Research area

Immunology

Target protein

IL-19

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE263 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000 pg/ml 2.754 2.677 2.579
2.6
1000 pg/ml 1.516 1.48 1.382
1.444
500 pg/ml 0.794 0.782 0.684
0.77
250 pg/ml 0.417 0.423 0.325
0.429
125 pg/ml 0.274 0.266 0.168
0.258
62.5 pg/ml 0.172 0.177 0.079
0.182
31.25 pg/ml 0.136 0.134 0.036
0.132
0 pg/ml 0.093 0.098 0
0.103

Precision

We measured random samples of BPE263 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 221 11.8 5.3
Intra-assay 2 20 515 23.5 4.6
Intra-assay 3 20 1470 30.3 2.1
Inter-assay 1 20 219 17 7.8
Inter-assay 2 20 512 40.2 7.9
Inter-assay 3 20 1465 93.1 6.4

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE263

    $2040.00

  • 96T

    BPE263

    $480.00

  • 15*96T

    BPE263

    $5400.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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