Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE349 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000 pg/ml	3.2	3.12	3.016
	3.041
1000 pg/ml	1.787	1.727	1.623
	1.668
500 pg/ml	1.008	0.956	0.852
	0.905
250 pg/ml	0.556	0.52	0.416
	0.485
125 pg/ml	0.319	0.303	0.199
	0.288
62.5 pg/ml	0.2	0.199	0.095
	0.199
31.25 pg/ml	0.162	0.152	0.048
	0.143
0 pg/ml	0.105	0.104	0
	0.104

We measured random samples of BPE349 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	237	6.1	2.6
Intra-assay	2	20	593	22.4	3.8
Intra-assay	3	20	1348	52.4	3.9
Inter-assay	1	20	236	19.4	8.2
Inter-assay	2	20	594	47.3	8
Inter-assay	3	20	1358	80.3	5.9

> Protocol Booklet

• Mouse Intercellular Adhesion Molecule 1, Icam1 Primacu™ ELISA Kit