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Home > Product > ELISA Primacu > Human Retinol-Binding Protein 4, RBP4 Primacu™ ELISA Kit

Human Retinol-Binding Protein 4, RBP4 Primacu™ ELISA Kit

Human Retinol-Binding Protein 4, RBP4 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 4.31 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 4.31 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Retinol-binding protein 4

Code

BPE070

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

4.31 pg/mL

Detection range

23.44-1500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM).

UniProt accession

MASS(Da)

23010

GeneID

5950

Gene names

RBP4

Research area

Cardiovascular,Metabolism,Signal transduction,Developmental biology

Target protein

RBP4

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE070 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1500 pg/ml 2.324 2.384 2.298
2.443
750 pg/ml 1.398 1.355 1.269
1.312
375 pg/ml 0.725 0.752 0.666
0.778
187.5 pg/ml 0.421 0.407 0.321
0.393
93.75 pg/ml 0.268 0.253 0.167
0.237
46.875 pg/ml 0.176 0.174 0.088
0.172
23.4375 pg/ml 0.133 0.134 0.048
0.134
0 pg/ml 0.082 0.086 0
0.09

Precision

We measured random samples of BPE070 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 142 4.6 3.3
Intra-assay 2 20 407 11.1 2.7
Intra-assay 3 20 1100 26.4 2.4
Inter-assay 1 20 142 8.8 6.2
Inter-assay 2 20 411 19.7 4.8
Inter-assay 3 20 1104 52.9 4.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE070

    $2040.00

  • 96T

    BPE070

    $480.00

  • 15*96T

    BPE070

    $5400.00

Add to Cart

Lead time: Within two weeks

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