Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE197 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1500 pg/ml	2.398	2.422	2.305
	2.446
750 pg/ml	1.168	1.218	1.101
	1.267
375 pg/ml	0.689	0.662	0.545
	0.635
187.5 pg/ml	0.373	0.383	0.266
	0.393
93.75 pg/ml	0.241	0.238	0.121
	0.234
46.875 pg/ml	0.173	0.181	0.064
	0.188
23.4375 pg/ml	0.152	0.15	0.033
	0.148
0 pg/ml	0.119	0.117	0
	0.115

We measured random samples of BPE197 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	175	10.6	6
Intra-assay	2	20	372	13.4	3.6
Intra-assay	3	20	1033	45.5	4.4
Inter-assay	1	20	173	16.2	9.4
Inter-assay	2	20	368	18	4.9
Inter-assay	3	20	1034	47.7	4.6

> Protocol Booklet