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Home > Product > ELISA Primacu > Human Macrophage Colony-Stimulating Factor 1, CSF1 Primacu™ ELISA Kit

Human Macrophage Colony-Stimulating Factor 1, CSF1 Primacu™ ELISA Kit

Human Macrophage Colony-Stimulating Factor 1, CSF1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 9.41 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 9.41 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Macrophage colony-stimulating factor 1

Code

BPE226

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

9.41 pg/mL

Detection range

23.44-1500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

UniProt accession

MASS(Da)

60179

GeneID

1435

Gene names

CSF1

Research area

Cardiovascular,Immunology,Neuroscience,Stem cells

Target protein

M-CSF

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE226 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1500 pg/ml 2.257 2.232 2.114
2.208
750 pg/ml 1.242 1.232 1.114
1.222
375 pg/ml 0.695 0.682 0.564
0.67
187.5 pg/ml 0.406 0.413 0.295
0.42
93.75 pg/ml 0.26 0.263 0.145
0.267
46.875 pg/ml 0.189 0.198 0.08
0.208
23.4375 pg/ml 0.165 0.161 0.043
0.158
0 pg/ml 0.115 0.118 0
0.121

Precision

We measured random samples of BPE226 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 64.8 4.8 7.4
Intra-assay 2 20 158 6.5 4.1
Intra-assay 3 20 791 30.5 3.9
Inter-assay 1 20 64.4 4.5 7
Inter-assay 2 20 157 11.1 7.1
Inter-assay 3 20 790 57.3 7.3

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE226

    $2040.00

  • 96T

    BPE226

    $480.00

  • 15*96T

    BPE226

    $5400.00

Add to Cart

Lead time: Within two weeks

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