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Home > Product > ELISA Primacu > Human Proto-oncogene c-Kit, KIT Primacu™ ELISA Kit

Human Proto-oncogene c-Kit, KIT Primacu™ ELISA Kit

Human Proto-oncogene c-Kit, KIT Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 6.98 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 6.98 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Mast/stem cell growth factor receptor Kit

Code

BPE238

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

6.98 pg/mL

Detection range

23.44-1500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family, and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

UniProt accession

MASS(Da)

109865

GeneID

3815

Synonyms

SCFR

Gene names

KIT

Research area

Cancer,Stem Cells,Signal transduction,Tags & cell markers,Developmental biology,Immunology,Neuroscience

Target protein

c-Kit

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE238 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1500 pg/ml 2.663 2.741 2.648
2.819
750 pg/ml 1.482 1.47 1.377
1.459
375 pg/ml 0.759 0.746 0.653
0.734
187.5 pg/ml 0.411 0.398 0.305
0.386
93.75 pg/ml 0.24 0.25 0.157
0.26
46.875 pg/ml 0.165 0.169 0.076
0.174
23.4375 pg/ml 0.127 0.134 0.041
0.142
0 pg/ml 0.1 0.093 0
0.087

Precision

We measured random samples of BPE238 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 125 5 4
Intra-assay 2 20 593 14.8 2.5
Intra-assay 3 20 1187 37.4 3.2
Inter-assay 1 20 124 10.2 8.2
Inter-assay 2 20 591 47.8 8.1
Inter-assay 3 20 1174 72.3 6.2

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE238

    $2040.00

  • 96T

    BPE238

    $480.00

  • 15*96T

    BPE238

    $5400.00

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Lead time: Within two weeks

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