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Home > Product > ELISA Primacu > Human Receptor Tyrosine-Protein Kinase Erbb-2, ERBB2 Primacu™ ELISA Kit

Human Receptor Tyrosine-Protein Kinase Erbb-2, ERBB2 Primacu™ ELISA Kit

Human Receptor Tyrosine-Protein Kinase Erbb-2, ERBB2 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 7.59 pg/mL

Detection range: 24-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 7.59 pg/mL

Detection range: 24-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Receptor tyrosine-protein kinase erbB-2

Code

BPE368

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

7.59 pg/mL

Detection range

24-1500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

UniProt accession

MASS(Da)

137910

GeneID

2064

Synonyms

HER2;MLN19;NEU;NGL

Gene names

ERBB2

Research area

Cancer,Neuroscience,Signal transduction,Tags & cell markers

Target protein

Her2

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE368 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1500 pg/ml 2.481 2.547 2.454
2.613
750 pg/ml 1.405 1.342 1.249
1.28
375 pg/ml 0.697 0.74 0.647
0.783
187.5 pg/ml 0.41 0.406 0.313
0.403
93.75 pg/ml 0.249 0.25 0.157
0.252
46.875 pg/ml 0.171 0.166 0.073
0.161
23.4375 pg/ml 0.119 0.127 0.034
0.135
0 pg/ml 0.09 0.093 0
0.096

Precision

We measured random samples of BPE368 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 53.7 1.7 3.1
Intra-assay 2 20 268 11.2 4.2
Intra-assay 3 20 994 33.4 3.4
Inter-assay 1 20 53.3 5 9.3
Inter-assay 2 20 267 17.8 6.7
Inter-assay 3 20 990 83.4 8.4

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE368

    $2040.00

  • 96T

    BPE368

    $480.00

  • 15*96T

    BPE368

    $5400.00

Add to Cart

Lead time: Within two weeks

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