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Home > Product > ELISA Primacu > Human C-C Motif Chemokine 14, CCL14 Primacu™ ELISA Kit

Human C-C Motif Chemokine 14, CCL14 Primacu™ ELISA Kit

Human C-C Motif Chemokine 14, CCL14 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 4.16 pg/mL

Detection range: 18.75-1200 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 4.16 pg/mL

Detection range: 18.75-1200 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

C-C motif chemokine 14

Code

BPE092

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

4.16 pg/mL

Detection range

18.75-1200 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

CCL14 has an important biological role in other mammals by evolving under positive selection that has been lost in Ochotonidae (subgenera Pika and Lagotona). CC chemokine ligand 14, CCL14, is a human CC chemokine that is of recent interest because of its natural ability, upon proteolytic processing of the first eight NH2-terminal residues, to bind to and signal through the human immunodeficiency virus type-1 (HIV-1) co-receptor, CC chemokine receptor 5 (CCR5). Embryo implantation is a complex process involving blastocyst attachment to the endometrial epithelium and subsequent trophoblast invasion of the decidua. We have previously shown that the chemokines CX3CL1 and CCL14 are abundant in endometrial vasculature, epithelial, and decidual cells at this time, and that their receptors, CX3CR1 and CCR1, are present on invading human trophoblasts. CX3CL1 and CCL14 promote trophoblast migration.

UniProt accession

MASS(Da)

10678

GeneID

6358

Synonyms

NCC2;SCYA14

Gene names

CCL14

Research area

Immunology,Metabolism,Signal transduction

Target protein

CCL14

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE092 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200 pg/ml 2.671 2.683 2.55
2.696
600 pg/ml 1.664 1.702 1.569
1.741
300 pg/ml 0.924 0.926 0.793
0.929
150 pg/ml 0.488 0.498 0.365
0.509
75 pg/ml 0.297 0.312 0.179
0.328
37.5 pg/ml 0.214 0.217 0.084
0.221
18.75 pg/ml 0.172 0.168 0.035
0.164
0 pg/ml 0.124 0.133 0
0.142

Precision

We measured random samples of BPE092 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 55.7 3.6 6.5
Intra-assay 2 20 253 7.1 2.8
Intra-assay 3 20 703 18.2 2.6
Inter-assay 1 20 55.5 5.5 9.9
Inter-assay 2 20 254 19.5 7.7
Inter-assay 3 20 698 43.1 6.2

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE092

    $2040.00

  • 96T

    BPE092

    $480.00

  • 15*96T

    BPE092

    $5400.00

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Lead time: Within two weeks

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