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Home > Product > ELISA Primacu > Human Nectin-2, NECTIN2 Primacu™ ELISA Kit

Human Nectin-2, NECTIN2 Primacu™ ELISA Kit

Human Nectin-2, NECTIN2 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 4.04 pg/mL

Detection range: 15.625-1000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 4.04 pg/mL

Detection range: 15.625-1000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Nectin-2

Code

BPE005

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

4.04 pg/mL

Detection range

15.625-1000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.

UniProt accession

MASS(Da)

57742

GeneID

5819

Synonyms

HVEB;PRR2;PVRL2

Gene names

NECTIN2

Research area

Immunology,Microbiology

Target protein

Nectin-2

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE005 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1000 pg/ml 2.212 2.204 2.094
2.195
500 pg/ml 1.26 1.238 1.128
1.215
250 pg/ml 0.69 0.707 0.597
0.723
125 pg/ml 0.4 0.408 0.298
0.415
62.5 pg/ml 0.253 0.268 0.158
0.283
31.25 pg/ml 0.187 0.189 0.079
0.19
15.625 pg/ml 0.156 0.149 0.039
0.141
0 pg/ml 0.112 0.11 0
0.107

Precision

We measured random samples of BPE005 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 137 8.7 6.4
Intra-assay 2 20 369 24.1 6.5
Intra-assay 3 20 786 38.8 4.9
Inter-assay 1 20 136 10.3 7.6
Inter-assay 2 20 367 23 6.3
Inter-assay 3 20 793 57.6 7.3

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE005

    $2320.00

  • 96T

    BPE005

    $580.00

  • 15*96T

    BPE005

    $6525.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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