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Home > Product > ELISA Primacu > Human Alpha-Fetoprotein, AFP Primacu™ ELISA Kit

Human Alpha-Fetoprotein, AFP Primacu™ ELISA Kit

Human Alpha-Fetoprotein, AFP Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 5.21 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 5.21 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Alpha-fetoprotein

Code

BPE245

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

5.21 pg/mL

Detection range

15.63-1000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD). Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

UniProt accession

MASS(Da)

68678

GeneID

174

Synonyms

HPAFP

Gene names

AFP

Research area

Cancer,Cardiovascular,Developmental biology

Target protein

Alpha-fetoprotein

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE245 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1000 pg/ml 2.483 2.6 2.519
2.717
500 pg/ml 1.371 1.438 1.357
1.505
250 pg/ml 0.748 0.74 0.659
0.732
125 pg/ml 0.443 0.423 0.342
0.403
62.5 pg/ml 0.23 0.241 0.16
0.252
31.25 pg/ml 0.164 0.155 0.074
0.147
15.625 pg/ml 0.111 0.108 0.027
0.105
0 pg/ml 0.076 0.081 0
0.086

Precision

We measured random samples of BPE245 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 31.1 1.7 5.5
Intra-assay 2 20 148 3.7 2.5
Intra-assay 3 20 511 12.2 2.4
Inter-assay 1 20 31.5 3.2 10.1
Inter-assay 2 20 146 14.5 9.9
Inter-assay 3 20 512 47.4 9.2

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 5*96T

    BPE245

    $2040.00

  • 96T

    BPE245

    $480.00

  • 15*96T

    BPE245

    $5400.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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