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Home > Product > ELISA Primacu > Human Pancreatic Triacylglycerol Lipase, PNLIP Primacu™ ELISA Kit

Human Pancreatic Triacylglycerol Lipase, PNLIP Primacu™ ELISA Kit

Human Pancreatic Triacylglycerol Lipase, PNLIP Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 4.27 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 4.27 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Pancreatic triacylglycerol lipase

Code

BPE274

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

4.27 pg/mL

Detection range

15.63-1000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

PNLIP is an enzyme that belongs to the lipase family. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.

UniProt accession

MASS(Da)

51157

GeneID

5406

Gene names

PNLIP

Research area

Cancer,Cardiovascular,Metabolism,Signal transduction

Target protein

PNLIP

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE274 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1000 pg/ml 2.275 2.172 2.041
2.068
500 pg/ml 1.314 1.277 1.146
1.24
250 pg/ml 0.732 0.713 0.582
0.694
125 pg/ml 0.44 0.421 0.29
0.402
62.5 pg/ml 0.255 0.265 0.134
0.274
31.25 pg/ml 0.196 0.189 0.058
0.181
15.625 pg/ml 0.167 0.159 0.028
0.151
0 pg/ml 0.125 0.131 0
0.136

Precision

We measured random samples of BPE274 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 91.5 4.9 5.3
Intra-assay 2 20 247 7.7 3.1
Intra-assay 3 20 727 41.6 5.7
Inter-assay 1 20 91 3.2 3.5
Inter-assay 2 20 245 20 8.2
Inter-assay 3 20 720 25 3.5

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE274

    $2320.00

  • 96T

    BPE274

    $580.00

  • 15*96T

    BPE274

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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