Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE289 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1000 pg/ml	2.635	2.517	2.408
	2.399
500 pg/ml	1.372	1.393	1.284
	1.414
250 pg/ml	0.8	0.773	0.664
	0.745
125 pg/ml	0.447	0.441	0.332
	0.434
62.5 pg/ml	0.273	0.279	0.17
	0.284
31.25 pg/ml	0.185	0.191	0.082
	0.197
15.625 pg/ml	0.165	0.156	0.047
	0.147
0 pg/ml	0.107	0.109	0
	0.111

We measured random samples of BPE289 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	151	3.2	2.1
Intra-assay	2	20	315	8.1	2.6
Intra-assay	3	20	787	24.3	3.1
Inter-assay	1	20	150	9.5	6.3
Inter-assay	2	20	316	15.8	5
Inter-assay	3	20	778	47	6

> Protocol Booklet

• Human Interleukin-13 Receptor Subunit Alpha-1, IL13RA1 Primacu™ ELISA Kit