Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE302 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1000 pg/ml	2.791	2.694	2.607
	2.597
500 pg/ml	1.382	1.377	1.29
	1.372
250 pg/ml	0.71	0.757	0.67
	0.804
125 pg/ml	0.431	0.429	0.342
	0.427
62.5 pg/ml	0.256	0.26	0.173
	0.265
31.25 pg/ml	0.183	0.174	0.087
	0.165
15.625 pg/ml	0.125	0.124	0.037
	0.123
0 pg/ml	0.088	0.087	0
	0.086

We measured random samples of BPE302 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	74.4	6.9	9.3
Intra-assay	2	20	257	25.5	9.9
Intra-assay	3	20	597	57.8	9.7
Inter-assay	1	20	74.9	8.4	11.2
Inter-assay	2	20	254	27.2	10.7
Inter-assay	3	20	592	53.1	9

> Protocol Booklet

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