Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE222 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800 pg/ml	2.115	2.205	2.086
	2.294
400 pg/ml	1.213	1.201	1.082
	1.188
200 pg/ml	0.708	0.665	0.546
	0.622
100 pg/ml	0.384	0.38	0.261
	0.376
50 pg/ml	0.246	0.252	0.133
	0.257
25 pg/ml	0.187	0.192	0.073
	0.197
12.5 pg/ml	0.171	0.164	0.045
	0.157
0 pg/ml	0.115	0.119	0
	0.123

We measured random samples of BPE222 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	41	3.8	9.2
Intra-assay	2	20	105	10.5	10
Intra-assay	3	20	420	41	9.8
Inter-assay	1	20	40.8	4.5	11.1
Inter-assay	2	20	104	11.3	10.8
Inter-assay	3	20	418	38.4	9.2

> Protocol Booklet